A premixed, ready-to-use solution containing all reagents required for hot-start multiplex PCR - just add your template DNA, primers and water. A Load-Ready master mix, this product includes an additional compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) to allow easy progress monitoring during electrophoresis. Generated PCR products are compatible with T/A cloning procedures. 1 ml vial Sufficient for 250 reactions at 4 μl master mix per reaction (i.e., 20 μl total reaction volume) Inhibits primer dimer formation Hot-start: initial activation in 12-15 min Multiplexing: tested with up to 18 targets (depending on amplicon length) Amplicon size: up to 5 kb Stable formulation may be shipped or temporarily stored for up to 1 month at room temperature, or up to 1 year at -20°C