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Each antibody delivers a strong signal with no cross-reactivity, and the dyes can be combined for two- or three-color Western blotting—for example, using anti-rabbit SpectraDye 650, anti-rat SpectraDye 550, and anti-mouse SpectraDye 490 together on a single blot. 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Each antibody delivers a strong signal with no cross-reactivity, and the dyes can be combined for two- or three-color Western blotting—for example, using anti-rabbit SpectraDye 650, anti-rat SpectraDye 550, and anti-mouse SpectraDye 490 together on a single blot. 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