This lab (formerly known as "miniPCR Antibiotic Resistance Lab: Monitoring resistant organisms in the environment") presents a fictional case study of a very real problem. Epidemiologists have traced an outbreak of antibiotic-resistant bacteria to one farm. Students will use PCR to determine if the antibiotic-resistant bacteria have spread to neighboring areas. This case study introduces students to how molecular analyses can serve as a tool in environmental monitoring and surveillance. Produced in collaboration with PARE – The Prevalence of Antibiotic-Resistance in the Environment project at Tufts University. No harmful samples are used in this lab.