4E5E2-1 (mouse lgG1/kappa) is specific to NC1 domain of a3 (IV).
Alport's syndrome, an inherited disease, shows absence or reduction of the a5 chain in the glomerular, tubular and bowman's capsular basement membranes. Normal human kidney has a1 (TV) to a6 (IV) chains in the renal basement membrance.
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