Total cell normalization and simultaneous detection of two biomarkers in whole cells.

AdvanCell in-cell Western Kit includes all critical reagents required to generate high-sensitivity whole-cell staining data for five 96-well plates. AdvanCell Permeabilization Solution removes membrane lipids (including the nuclear membrane) to let antibodies enter the cell and detect nuclear proteins, while AdvanCell Total Cell Staining Solution is a nuclear stain provided as a 500X solution intended to be co-incubated with primary antibodies to save time and reduce assay steps. Each kit includes one SpectraDye IR700- and one SpectraDye IR800-conjugated secondary antibody directed against mouse or rabbit primary antibodies, allowing simultaneous detection of two biomarkers directly in cells with minimal sample processing.

Advantages:
• Saves time – cells are directly probed, no time-consuming lysate preparation needed
• Quantitative immunofluorescence – normalize to cell number for accurate quantification and comparison between wells
• Detects high and low molecular weight targets simultaneously, unlike traditional Western blots
• Detects proteins in situ in the most relevant cellular context with minimal sample processing