{"product_id":"advancell-in-cell-western-kit-rabbit-ir700-mouse-ir800","title":"AdvanCell In Cell Western Kit, Rabbit IR700\/Mouse IR800","description":"\u003cp\u003eTotal cell normalization and simultaneous detection of two biomarkers in whole cells.\u003c\/p\u003e\n\n\u003cp\u003eAdvanCell in-cell Western Kit includes all critical reagents required to generate high-sensitivity whole-cell staining data for five 96-well plates. AdvanCell Permeabilization Solution removes membrane lipids (including the nuclear membrane) to let antibodies enter the cell and detect nuclear proteins, while AdvanCell Total Cell Staining Solution is a nuclear stain provided as a 500X solution intended to be co-incubated with primary antibodies to save time and reduce assay steps. Each kit includes one SpectraDye IR700- and one SpectraDye IR800-conjugated secondary antibody directed against mouse or rabbit primary antibodies, allowing simultaneous detection of two biomarkers directly in cells with minimal sample processing.\u003c\/p\u003e\n\n\u003cp\u003eAdvantages:\u003cbr\u003e\n• Saves time – cells are directly probed, no time-consuming lysate preparation needed\u003cbr\u003e\n• Quantitative immunofluorescence – normalize to cell number for accurate quantification and comparison between wells\u003cbr\u003e\n• Detects high and low molecular weight targets simultaneously, unlike traditional Western blots\u003cbr\u003e\n• Detects proteins in situ in the most relevant cellular context with minimal sample processing\u003c\/p\u003e","brand":"Advansta","offers":[{"title":"assay plate","offer_id":49295640559872,"sku":"K-15065-005","price":885.28,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0663\/6591\/5392\/files\/Advansta_78a79086-59d0-409c-b3bc-4b6f10f451d8.png?v=1787518602","url":"https:\/\/shop.customscience.co.nz\/products\/advancell-in-cell-western-kit-rabbit-ir700-mouse-ir800","provider":"Custom Science Ltd","version":"1.0","type":"link"}