The E-Z 96 Total RNA Kit is designed for isolation of total cellular RNA from up to 5 x 106 cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilizing HiBind silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind RNA Plate where the RNA is purified via three wash steps. High-quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96 Total RNA method is ready for applications such as RTPCR,qPCR, differential display, microarrays, and other downstream applications.