The E.Z.N.A. High Performance (HP) Fungal DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from fresh and dried fungal tissue samples rich in polysaccharides or having lower DNA content. Up to 100 mg wet tissue (or 30 mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.