The E.Z.N.A. Micro RNA Kit combines the reversible binding properties of HiBind matrix, a new silica-based material, with the highly efficient lysis ability of RNA-Solv to extract micro and large (>200 nt) RNA from a wide variety of starting materials. Micro and large RNA are separated to allow for quantification into downstream applications. The system uses RNA-Solv to efficiently lyse cells and protect RNA from degradation. Following a chloroform separation step, RNA is application to a HiBind RNA column where large RNA binds. The flow through is retained and applied to a HiBind Micro RNA column to bind small RNA