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E.Z.N.A.® Endo-free Plasmid DNA Mini Kit I

SKU D6948-00
Original price $38.79 - Original price $140.17
Original price
$38.79
$38.79 - $140.17
Current price $38.79
Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. Up to 5 mL of overnight cultures can be used for high copy number plasmids and 10 mL for low copy number. The E.Z.N.A. Endo-Free Plasmid Mini Kit I integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0.1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind DNA column and purified DNA is eluted from the column membrane.

Delivery time

Overnight if in stock

Payment Methods

Purchase orders from approved customers and Credit Cards are accepted as forms of payment

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