The E.Z.N.A. Plasmid Mini Kit I is designed to isolate up to 30 µg of high-quality plasmid DNA from 1-5 mL bacterial cultures in 30 minutes or less. Plasmid DNA purification is simplified with HiBind Mini Column technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, and manual/automated fluorescent DNA sequencing. E.Z.N.A. Plasmid Mini Kit I is available in V- Spin and Q-Spin column formats. V Spin Column Formats have an attached lid while Q Spin Columns do not. Both Versions can be used for centrifugation or vacuum protocols.