E.Z.N.A. MicroElute Total RNA Kit provides a rapid and easy method for the isolation of up to 50 �g of total RNA from small amounts of cultured eukaryotic cells, tissues such as as laser dissected samples (LDS) or fine needle aspirates(FNA). Normally, up to 5 x 10^5 eukaryotic cells or 5 mg tissue can be used in a single experiment depending on the type of tissue used. This kit allows processing of single or multiple of samples in less than 30 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifuation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10-15 �L nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly A+ purification, Nuclease protection and in vitro translation.