IsoFast™ Bst Polymerase is a recombinant form of the large fragment of Bst DNA polymerase, containing 5′-3′ polymerase activity and strand displacement activity.
The enzyme offers fast amplification and strong strand displacement capabilities, making it ideal for nucleic acid amplification methods such as whole genome amplification, multiple displacement amplification and isothermal amplification.
Has strand-displacing 5′-3′ polymerase activity
Lacks 5′-3′ exonuclease activity
DNA synthesis is performed at a constant temperature
Operates over a broad temperature range, with an optimum of 65ºC
Gives rapid and consistent amplification across a wide range of templates
Supplied with an advanced 2-part buffer system for higher yields under difficult conditions
30 minute protocol
Flexible formats with and without fluorescent dye
Also available as a 2x mix and as a dual enzyme 1-step system for RNA amplification