LBA4404 is an established Agrobacterium tumefaciens strain for binary-vector delivery in stable and transient plant transformation workflows. The methionine-auxotrophic format requires methionine supplementation, providing a nutritional dependency that can help control bacterial persistence after plant co-cultivation; cells are prepared for electroporation. It is supplied as 5x50µl, stored at -80°C, and has a stated shelf life of ≥ 15 months.