One vial of lyophilized E. colicells, optional add-on to the Knockout! CRISPR/Cas Lab. In the Knockout! Lab, bacteria are transformed with the pKO plasmid (encoding Cas9 and a guide RNA that targets the lacZ gene) or with the pCtrl plasmid (encoding Cas9 and a random guide RNA). Once reconstituted in LB-Amp liquid media, the lyophilized bacteria must be transformed within 48-72 hours. Adding a second vial of cells allows you to carry out transformations on different days with different lab groups, thus making it feasible to split the kit into lab groups working on different weeks.   Please refer to the Knockout! Lab Instructor's Guide for detailed instrucions. This product is sold for educational use only. It contains technologies that are owned and licensed for educational purposes only by miniPCR bio.