Illuminate gene expression with the BioBits cell-free system. This refill minikit contains reagents for two lab groups. BioBits pellets are tiny molecular factories that can create proteins without the need for cell culture. When dry, BioBits pellets are dormant, but they can be activated by simply adding water. Researchers have been using cell-free reactions in their laboratories for years, with applications ranging from novel therapeutic discovery to field diagnostics. Now the BioBits cell-free system makes this cutting-edge technology accessible to anyone interested in learning molecular biology. With minimal equipment requirements and a quick and straightforward protocol, students will use the BioBits system to visualize the flow of genetic information and monitor transcription and translation in real-time through fluorescence. This activity serves as an excellent interactive tool for learning molecular biology’s central dogma and exposes students to cutting-edge synthetic biology.