This lab demonstrates the power that molecular techniques bring to managing infectious disease outbreaks. In this case study students act as healthcare providers at an airport screening facility and test fictional patients for infection with the SARS-CoV-2 virus. Students use PCR and a fluorescence viewer to diagnose their patients.   The data for this lab can be collected two ways: endpoint detection or qPCR time point observations. Endpoint detection allows your students to use a single observation of fluorescence to diagnose their patients, in a single class period and without the need to run a gel . For longer classes, students can monitor their PCR samples over time to model the principles of qPCR, the gold standard for diagnosis of SARS-CoV-2 infection. Techniques: Micropipetting, qPCR, fluorescence visualization, gel electrophoresis (optional) Topics: Infectious disease, molecular diagnostics, biotechnology Time required: One or two class periods (depending on approach) Level: General high school, advanced high school, college Disclaimer: no pathogenic materials are used. This experimental protocol engages students in a simulated patient diagnosis exercise. None of the materials provided in this lab kit pose a pathogenic risk. For educational use only. Not for diagnostic use.